ABSTRACT
Objective:To explore the expression and clinical significance of S100A8 and S100A9 in Helicobacter pylori ( H. pylori) associated gastritis. Methods:A total of 101 patients with chronic gastritis diagnosed in the First Hospital of Shanxi Medical University from October 2018 to May 2019 were selected. The expression levels of S100A8 and S100A9 in the gastric mucosa tissues of 101 patients with chronic gastritis were determined by immunohistochemistry (in absorbance), and the mRNA expression levels of S100 A8 and S100 A9 in the gastric mucosa tissues of 48 patients were detected by reverse transcription-polymerase chain reaction. And the results combined with pathological diagnosis of routine staining and clinical H. pylori infection data were analyzed. Mann-Whitney U test, Kruskal-Wallis H test and Spearman rank correlation were used for statistical analysis. Results:Among 101 patients, there were 59 cases of chronic atrophic gastritis (CAG group) and 42 cases of chronic non-atrophic gastritis (NAG group); 59 cases were H. pylori positive ( H. pylori positive group) and 42 cases were H. pylori negative ( H. pylori negative group). There were statistically significant differences in the expression levels of S100A8 and S100A9 between CAG group and NAG group (0.10, 0.07 to 0.13 vs. 0.09, 0.06 to 0.10 and 0.13, 0.08 to 0.15 vs. 0.09, 0.07 to 0.10, respectively), and between H. pylori positive group and H. pylori negative group (0.11, 0.10 to 0.13 vs. 0.07, 0.06 to 0.08 and 0.13, 0.10 to 0.15 vs. 0.07, 0.07 to 0.08, respectively) ( U=754.00, 602.00, 5.00 and 40.00, all P<0.01). There were statistically significant differences in the expression levels of S100A8 and S100A9 between H. pylori positive patients (34 cases) and H. pylori negative patients (25 cases) in CAG group (0.13, 0.11 to 0.14 vs. 0.07, 0.07 to 0.08 and 0.15, 0.14 to 0.16 vs. 0.08, 0.08 to 0.09, respectively), similarly, there were significant differences in the expression levels of S100A8 and S100A9 between H. pylori positive patients (25 cases) and H. pylori negative patients (17 cases) in NAG group (0.10, 0.09 to 0.10 vs. 0.06, 0.05 to 0.07 and 0.10, 0.10 to 0.11 vs. 0.07, 0.06 to 0.07, respectively) ( U=1.00, 0.00, 0.00 and 0.00, all P<0.01). The results indicated that the expression levels of S100A8 and S100A9 were high in H. pylori positive patients in CAG group, the expression levels of S100A8 and S100A9 were low in H. pylori negative patients in NAG group, and the differences were statistically significant ( H=84.78 and 89.64, both P<0.01). There were statistically significant differences in the expression of S100 A8 and S100 A9 at mRNA level between CAG group (24 cases) and NAG group (24 cases) (0.12, 0.06 to 1.31 vs. 0.05, 0.03 to 0.08; 0.19, 0.03 to 0.43 vs. 0.03, 0.01 to 0.09), and the expression of S100 A8 and S100 A9 at mRNA level was significant between H. pylori positive patients (24 cases) and H. pylori negative patients (24 patients) (0.45, 0.10 to 1.90 vs. 0.05, 0.03 to 0.08 and 0.36, 0.24 to 0.81 vs. 0.03, 0.01 to 0.04) ( U=55.00, 74.00, 19.00 and 2.00, all P<0.05). There were statistically significant differences in the expression of S100 A8 and S100 A9 at mRNA level between H. pylori positive patients (12 cases) and H. pylori negative patients (12 cases) of CAG group (0.85, 0.27 to 2.28 vs. 0.06, 0.03 to 0.09 and 0.39, 0.25 to 0.87 vs. 0.03, 0.02 to 0.05), and the expression of S100 A8 and S100 A9 at mRNA level was significant between H. pylori positive patients (12 cases) and H. pylori negative patients (12 cases) of NAG group (0.09, 0.05 to 0.28 vs. 0.04, 0.03 to 0.07 and 0.20, 0.09 to 0.65 vs. 0.01, 0.01 to 0.03) ( U=5.00, 2.00, 0.00 and 0.00, all P<0.01). The results showed that the expression of S100 A8 and S100 A9 at mRNA level was high in H. pylori positive patients in CAG group, the expression of S100 A8 and S100 A9 at mRNA level was low in H. pylori negative patients in NAG group, and the differences were statistically significant ( H=20.43 and 24.15, both P<0.01). The expression levels of S100A8 and S100A9 were positively correlated at both protein level and mRNA level ( r=0.899 and 0.903, both P<0.01). Conclusions:S100A8 and S100A9 may involve in the inflammation process of H. pylori-infected gastric mucosa and promote the proliferation of gastric epithelial cells, which may be one of mechanisms of intrinsic glands reduction and CAG genesis. S100A8 and S100A9 are expected to be potential biomarkers for diagnosis and follow-up and potential targets for treatmert of CAG.
ABSTRACT
Objective To study the changes and significance of serum neutrophil gelatinase associated li-pocalin (NGAL ) ,cyclooxygenase 2 (COX-2 ) and pepsinogen (PG ) in the patients with gastric cancer . Methods Sixty cases of gastric cancer in this hospital from April 2015 to April 2017 were selected as the ob-servation group ,and contemporaneous 60 healthy subjects were selected as the control group .The double-anti-body sandwich enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of serum NGAL , COX-2 ,PGⅠ ,PG Ⅱ and PG Ⅰ /PGⅡ (PGR) in the two group .The results were compared .The relationship between serum NGAL and COX-2 with the clinicopathological parameters of gastric cancer was analyzed . Results The levels of serum NGAL and COX-2 in the observation group were (23 .43 ± 8 .34)ng/mL and (41 .44 ± 9 .51)ng/mL respectively ,which were higher than (11 .73 ± 2 .81)ng/mL and (16 .89 ± 6 .26)ng/mL in the control group ,the difference was statistically significant (P<0 .05) .Serum PGⅠ and PGR levels in the observation group were(13 .07 ± 20 .19)ng/mL and (2 .69 ± 1 .41) ,which were lower than (60 .15 ± 18 .70) ng/mL and (5 .08 ± 1 .86) in the control group ,the difference was statistically significant (P<0 .05) .The ser-um NGAL level in the patients with TNM stage Ⅰ + Ⅱwas significantly lower than that in the patients with TNM stage Ⅲ + Ⅳ ,the difference was statistically significant (P<0 .05);the serum NGAL level in the pa-tients with distant metastasis was significantly higher than that in the patients without distant metastasis ,the difference was statistically significant (P<0 .05) .The serum COX-2 level in the patients with TNM stage Ⅰ +Ⅱwas significantly lower than that in the patients with TNM stage Ⅲ + Ⅳ ,the difference was statistically sig-nificant(P<0 .05) .Conclusion Serum NGAL ,COX-2 and PG can serve as the effective indicators for gastric cancer screening ,disease condition judgment and prognosis assessment in gastric cancer .